Fig 1: Pi increases VEGFA bioavailability(A) HCs were pretreated for 2 h with the pan-MMP inhibitor, MMP-V (10 μM) followed by treatment with Si (7 mM) or Pi (7 mM) for 10 min. ELISA was performed to quantitate VEGFA in the conditioned media. Data represent the mean ± SD; of that obtained from three to four independent chondrocyte preparations; p values are indicated above the line.(B) Western analyses of the treated HCs using anti-pERK1/2 & tERK1/2 antibodies. (C) Densitometric quantitation of western data in (B). Densitometric data represent mean ± SD; of that obtained from three to four independent chondrocyte preparations; p values are indicated above the line.
Supplier Page from Bioss Inc. for Mouse VEGF ELISA Kit